“Everyone should do research”




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POSTER PRESENTATION ABSTRACTS



P1

BECKMAN COULTER LH750 VCS PARAMETERS EVALUATED FOR SCREENING AND DETECTION OF ABNORMAL LYMPHOCYTE POPULATIONS




1P. Baron, 1K.Andriolo, 2L.Johnstone, 1, 3D Talaulikar.


1Department of Haematology, ACT Pathology, Canberra Hospital, PO Box 11, Woden ACT 2606. 2Beckman Coulter, Australia, 3ANU Medical School, Canberra.

kerrie.andriolo@act.gov.au


Introduction

The Beckman Coulter haematology analysers use Volume Conductivity and Scatter (VCS) measurements to differentiate between white cell populations. Recent studies have shown that mean and standard deviation (SD) of Volume Conductivity and Scatter (VCS) measurements can be used to enhance detection of abnormalities in patient leukocyte populations. This study examines the use of these parameters in detecting abnormal lymphocyte populations.


Methods

Blood samples from 53 normal patients and 124 patients with proven lymphocyte abnormalities were analysed on a Beckman Coulter LH 750 series automated analyser in the Department of Haematology, ACT Pathology. The mean and SD of VCS measurements on lymphocyte populations were analysed using MedCalc Statistical software Version 9.3.0.0, and receiver operating curves (ROC) were used to determine if parameters could have diagnostic value.


Results

Values, as compared with normal patients were found to be significantly different for volume SD (vSD), conductivity mean (cM) and conductivity SD (cSD) in most malignant conditions. In CLL, vSD, cM, and cSD clearly differentiated 95% of cases from normal patients. However, the measurements were not useful in separating different patient populations as there was significant overlap between the groups.


Conclusions

Volume SD (vSD), conductivity mean (cM) and conductivity SD (cSD) appear to have the potential to enhance the detection capability of abnormal lymphocytes in malignant conditions, particularly in chronic lymphocytic leukaemia.


Notes

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P2


ROLE OF IMMUNOHISTOCHEMISTRY IN STAGING DIFFUSE LARGE B-CELL LYMPHOMA (DLBCL)


1, 2D Talaulikar, 2, 3JE Dahlstrom, 2, 4B Shadbolt, 3A Broomfield, 5A McDonald


1Department of Haematology, 2Australian National University Medical School, 3Department of Anatomical Pathology, 4Department of Epidemiology, The Canberra Hospital, Canberra, ACT, Australia, 5National Capital Private Hospital, Canberra, ACT.

dipti.talaulikar@act.gov.au


Introduction

The use of immunohistochemistry in staging bone marrow in Non-Hodgkin’s Lymphoma (NHL) is largely limited to ambiguous cases, particularly those with lymphoid aggregates. Its’ role in routine clinical practice remains unestablished.


Aims

This study aimed to determine if the routine use of immunohistochemistry (IHC) in Diffuse Large B-cell lymphoma (DLBCL) would improve the detection of lymphomatous involvement in the bone marrow. It also sought to determine the impact of IHC on predicting survival as compared to routine histological diagnosis using Haematoxylin and Eosin (H&E), Giemsa and reticulin staining.


Methods and results

The bone marrow trephines of 156 histologically proven DLBCL cases were assessed on routine histology, and IHC using two T-cell markers (CD45RO and CD3), two B-cell markers (CD20 and CD79a) and Kappa and lambda light chains. IHC detected lymphomatous involvement on an additional 11% cases as compared to histology alone. Although both routine histology and IHC were good predictors of survival, IHC was better at predicting survival on stepwise multivariate Cox regression analysis.


Conclusions

Immunohistochemistry performed routinely on bone marrow trephines has the ability to improve detection of occult lymphoma in experienced hands. Furthermore, it is a better predictor of survival as compared to routine histological examination alone.


Notes

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P3


EndotheliAL P-Selectin versus Platelet P-Selectin in Tumour Cell Metastasis


LA Coupland, L. Hindmarsh, CR Parish.


The John Curtin School of Medical Research, Australian National University

Lucy.Coupland @anu.edu.au


Introduction

P-selectin, a member of the vascular adhesion receptor family, is expressed on platelets and endothelium, with previous studies implicating platelet P-selectin in tumour metastasis. The influence of endothelial P-selectin on the metastatic process, however, has not been elucidated. The aim of this study was to compare the roles of endothelial and platelet P-selectin (P-sel) in an in-vivo model of melanoma metastasis in P-selectin chimeric mice.


Methods

CD45.2+ male C57BL/6 mice (wild type or P-sel KO), and CD45.1+ B6.SJL/J mice were administered 1000 rads from a cobalt-60 source. Marrow infusions generated 3 experimental groups namely: (1) transplant controls, (2) platelet P-sel +ve/endothelium P-sel –ve, and (3) platelet P-sel –ve/endothelium P-sel +ve mice. Marrow engraftment was confirmed by leukocyte CD45.1 and CD45.2, and platelet P-sel expression. Four months following irradiation, all mice were injected IV with B16 melanoma cells, sacrificed 14 days later, lungs removed and metastases counted.


Results

CD45.1 vs CD45.2 measurements demonstrated ≥80% of leukocytes in each group were donor marrow derived. Platelet P-sel expression showed equivalent levels in transplant controls and platelet P-sel +ve/endothelium P-sel –ve mice, but low levels of residual expression in the platelet P-sel –ve/endothelium P-sel +ve mice.

Mean metastasis counts in the lungs of the 3 groups were as follows:- transplant controls 124 +/- SE 10.8, platelet P-sel +ve/endothelium P-sel –ve 38 +/- SE 6.2 (p=0.002) and platelet P-sel –ve/endothelium P-sel +ve 28 +/- SE 3.8 (p=0.0008).


Conclusions

The generation of P-selectin chimeric mice has enabled the study of the independent roles of endothelial and platelet P-selectin in an in-vivo model of melanoma metastasis. The results of this study demonstrate highly significant roles for both endothelium and platelet P-selectin in the metastatic process.


Notes

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P4


EFFECT OF AN EARLIER DISCHARGE OF PREMATURE INFANTS ON PARENT SATISFACTION


1M L Stilianos­­, 2Z Kecskés


1Medical Student Year 2, Australian National University Medical School, Canberra, ACT, Australia

2Consultant Neonatologist, Department of Neonatology, Canberra Hospital

Senior Lecturer, Neonatology, Australian National University, Canberra, ACT, Australia

PO Box 11 Woden

Neonatology Department, The Canberra Hospital, Australian National University Medical School, ACT Australia, 2606

u4289738@anu.edu.au


Introduction

Research on neonatal discharge programs has not focused on psychosocial issues. Long hospital stays can be detrimental to infants and parents, affecting bonding, emotional, financial and marital stress, family functioning and develop anxiety. In 2005, the Centre for Newborn Care at Canberra Hospital changed discharge guidelines so parents administered gavage feeds at home with neonatal nursing in-home support. Before, infants were discharged when all but two of daily feeds were suck feeds, these were given by neonatal nursing in-home support. The aim was to facilitate early discharge, enhance the neonatal health care approach and facilitate positive patient and family centred outcomes.


Methods

The control group were parents whose babies were discharged when taking all but two feeds as suck feeds; the study group were parents whose babies were discharged when they were able to take four suck feeds and four gavage feeds. A survey was mailed, with telephone follow-up and a second mail out of surveys.


Results

The response rate was 48%; 28 of the control group and 36 of the study group responded. There were no statistical significances. The study group responded more positively to bonding experiences at home and hospital, being relaxed in the home, the assurance of gavage feeds and appreciated both parents being able to feed, but were less satisfied with hospital support before discharge. The control group reported less positive experiences including difficulty transitioning to home and breastfeeding, the anxiety that ensured and minimal support received in these areas.


Conclusions

The results of this small study show similar satisfaction with two different gavage feeding regimens at discharge. The results indicate that an earlier discharge with gavage feeding presents with other positives of an earlier discharge and does not have a negative impact on parental satisfaction.


Notes:

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P5


Effect of indomethacin, ibuprofen and gentamicin on glomerular number in the neonatal rat.


1,7*AL Kent 2R Douglas-Denton, 3,7 B Shadbolt 4,7E Dahlstrom, 4LE Maxwell, 4ME Koina, 5MC Falk, 6,7D Willenborg, 2JF Bertram.


1Department of Neonatology, The Canberra Hospital, PO Box 11, Woden, ACT 2606, Australia, 2Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC, 3800, Australia, 3Clinical Epidemiology Unit, 4Department of Anatomical Pathology, 5Department of Nephrology, The Canberra Hospital, PO Box 11, Woden, ACT 2606, 6Neurosciences Research Unit, Australian National University, Canberra, ACT 2601. 7Australian National University Medical School, Canberra, ACT 2601.

alison.kent@act.gov.au


Background

Premature neonates are frequently administered indomethacin, ibuprofen and gentamicin during the period of active glomerulogenesis. These drugs are known to have nephrotoxic effects, but the morphological effect of these drugs is largely unknown.


Aim

To determine whether administration of these drugs during glomerulogenesis has an effect on glomerular endowment in a neonatal rat model.


Methods

Rat pups were administered intraperitoneal indomethacin, ibuprofen or indomethacin and gentamicin for 5 days postnatally from day 1 of postnatal life. The pups were sacrificed at 14 days of age at completion of glomerulogenesis. Following fixation, the total number of glomeruli was counted in the left kidney using the physical disector/fractionator stereological technique.


Results

There was no significant difference between treatment groups in the total number of glomeruli per kidney (p=0.45), but there were significantly less glomeruli per gram of kidney in those rat pups that had received indomethacin or ibuprofen (p<0.0001).


Discussion

The reduction in the number of glomeruli per gram of kidney may indicate altered nephron development, may be dose related or as a consequence of interstitial oedema secondary to the drugs. Further studies are required to determine whether this relative reduction in glomeruli persists and results in glomerulosclerosis in later life.


Notes:

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P6


Are renal volumes measured by magnetic resonance imaging and three-dimensional ultrasound in the term neonate comparable?


1,6AL Kent, 2R Jyoti, 2C Robertson, 2L Gonsalves, 3Sandra Meskell, 4,6 B Shadbolt, 5MC Falk.


1Dept of Neonatology, 2Dept of Medical Imaging, 3Clinical Research Nurse, Centre for Newborn Care, 4Clinical Epidemiology Unit, 5Dept of Nephrology, The Canberra Hospital, Canberra, ACT, Australia, 6Australian National University Medical School, Canberra, ACT.

alison.kent@act.gov.au


Introduction

Renal volume, but not renal length, has been shown to be positively correlated with renal function.


Aim

To determine whether 3D ultrasound measurements of renal volume in the neonate are comparable to those of MRI measurements.


Methods

Preterm and term neonates had an MRI and 3D ultrasound to determine renal volume at the same time as they were having an MRI brain for other clinical conditions. The preterm neonates were all term corrected age and the term neonates 1 to 4 weeks of age. None of the kidneys examined were abnormal.


Results

There were no significant differences in the weight or length of the preterm and term infants at the time of their MRI. The left renal length was significantly longer when measured by MRI than 3D ultrasound (p=0.02). Renal volumes of both left and right kidney were greater when measured by MRI than 3D ultrasound (p<0.0001 respectively). Total volumes of kidneys were greater when measured by MRI than 3D ultrasound (p=0.008).

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