In college of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
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| Fragaria spp. SO - Jiangsu Journal of Agricultural Sciences. 2008. 24: 6, 875-877. 7 ref. PU - Jiangsu Academy of Agricultural Sciences LP - Nanjing CP - China AB - A fast and efficient method for isolation of total RNA from leaves of strawberry (Fragaria spp.) in vitro and field was developed. The RNA samples of the leaves of strawberry were isolated by improved CTAB method, and the purity and quantity of each RNA obtained was evaluated by agarose gel electrophoresis, UV scanning and RT-PCR analysis. The total RNA of strawberry leaves extracted by this method showed clear hands of 28 S rRNA, 18 S rRNA and 5.8 S rRNA, and the values of OD260/OD280 were between 1.83 and 2.14. The cDNA reversely transcripted from the extracted RNA exhibited clear band by RT-PCR, which meant that the quality and purity of the RNA could meet the demands of molecular biology experiment. CC - Horticultural Crops [FF003] CC - Plant Breeding and Genetics [FF020] CC - Molecular Biology and Molecular Genetics [ZZ360] CC - Techniques and Methodology [ZZ900] MH - complementary DNA MH - electrophoresis MH - isolation techniques MH - leaves MH - ribosomal RNA MH - RNA MH - strawberries MH - Fragaria MH - Fragaria ananassa BT - Rosaceae BT - Rosales BT - dicotyledons BT - angiosperms BT - Spermatophyta BT - plants BT - eukaryotes BT - Fragaria ID - cDNA ID - ribonucleic acid ID - rRNA LG - Chinese SL - English PT - Journal article SS - 0C SS - 7B SS - 0P SS - CA SS - HO SS - PL SS - ZS UR - http://www.jaas.ac.cn IS - 1000-4440 UP - 20090408 YR - 2008 <83> VN - Ovid Technologies DB - CAB Abstracts UI - 20093086466 AU - Zhao TongMin AU - Yu WenGui AU - Fan XueZhen AU - Chen ZhiYi AU - Luo ChuPing IN - Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. MA - tmzhaomail@yahoo.com.cn TI - Identification and analysis of isozyme genotypes of Phytophthora infestans. SO - Jiangsu Journal of Agricultural Sciences. 2008. 24: 6, 871-874. 19 ref. PU - Jiangsu Academy of Agricultural Sciences LP - Nanjing CP - China AB - Four genotypes (100/100/111, 86/100/111, 86/100 and 100/100) of glucose-6-phosphate isomerase (Gpi) and 2 genotypes (100/100 and 92/100) of peptidase (Pep) of 91 strains of P. infestans were identified by cellulose acetate electrophoresis (CAE). 100/100/111 was the priority genotype of Gpi and 100/100 was the priority genotype of Pep, which accounted for 85.7% and 94.5% of the strains tested, respectively. CAE was a rapid and reliable approach for identification of isoenzyme genotypes of P. infestans. RN - 9004-35-7 RN - 9001-41-6 CC - Viral, Bacterial and Fungal Diseases of Plants [FF610] CC - Biochemistry and Physiology of Microorganisms [ZZ394] CC - Genetics and Molecular Biology of Microorganisms [ZZ395] CC - Techniques and Methodology [ZZ900] MH - cellulose acetate MH - electrophoresis MH - enzymes MH - genotypes MH - glucose-6-phosphate isomerase MH - isoenzymes MH - peptidases MH - plant pathogenic fungi MH - plant pathogens MH - techniques MH - fungi MH - Phytophthora infestans BT - Phytophthora BT - Peronosporales BT - Mastigomycotina BT - Eumycota BT - fungi BT - eukaryotes ID - isozymes ID - Peronosporomycetes ID - phosphoglucose isomerase ID - phosphohexose isomerase ID - phytopathogens ID - proteases ID - proteolytic enzymes ID - Pythiaceae LG - Chinese SL - English PT - Journal article SS - 0M SS - 7K SS - CA SS - PE SS - QI SS - ZS UR - http://www.jaas.ac.cn IS - 1000-4440 UP - 20090408 YR - 2008 <84> VN - Ovid Technologies DB - CAB Abstracts UI - 20093086465 AU - Fan Feng AU - Zhao ZhongXin AU - Ni GuYin AU - Mao AiMing AU - Cheng ZhiGuo AU - Lu ChunBing AU - Bi ZouSheng IN - Wuxi Center for Animal Disease Control and Prevention, Wuxi 214000, China. TI - Immune responses of chicken flocks to inactivated avian influenza vaccine in Wuxi area. SO - Jiangsu Journal of Agricultural Sciences. 2008. 24: 6, 867-870. 4 ref. PU - Jiangsu Academy of Agricultural Sciences LP - Nanjing CP - China AB - In this study, several flocks were immunized with vaccines against avian influenza serotype H5 or/and H9, for monitoring the antibody level of highly pathogenic avian influenza (HPAI). The results showed that H5-H9 bivalent inactivated vaccine was effective in the prevention of avian influenza in Wuxi district, China. Enhancing the detection of infection ratio in waterfowl and birds is the basis of preventing the virus from the flocks. It indicated that the immune procedure may he helpful for efficient control of the disease. CC - Host Resistance and Immunity [HH600] CC - Animal Immunology [LL650] CC - Prion, Viral, Bacterial and Fungal Pathogens of Animals [LL821] MH - antibodies MH - avian influenza MH - avian influenza A viruses MH - disease control MH - disease prevention MH - immune response MH - inactivated vaccines MH - poultry MH - vaccination MH - fowls MH - Influenza A virus MH - China BT - Orthomyxoviridae BT - negative-sense ssRNA viruses BT - ssRNA viruses BT - RNA viruses BT - viruses BT - East Asia BT - Asia BT - Developing Countries BT - Gallus gallus BT - Gallus BT - Phasianidae BT - Galliformes BT - birds BT - vertebrates BT - Chordata BT - animals BT - poultry BT - eukaryotes BT - Influenzavirus A ID - Avian influenzavirus ID - bird flu ID - bird grippe ID - bird influenza ID - chickens ID - domesticated birds ID - immunity reactions ID - immunological reactions ID - Influenzavirus ID - killed vaccines LG - Chinese SL - English PT - Journal article SS - 7A SS - 0I SS - 0V SS - CA SS - VE SS - ZA SS - QV SS - QI SS - ZS UR - http://www.jaas.ac.cn IS - 1000-4440 UP - 20090408 YR - 2008 <85> VN - Ovid Technologies DB - CAB Abstracts UI - 20093086463 AU - Xu XiangMing AU - Wang Lin AU - Zhai GuoQin AU - Gong Wen AU - Hua Zheng AU - Chen Bing AU - Xue ZhengFeng IN - Jiangsu College of Animal Husbandry & Veterinary, Taizhou 225300, China. TI - Dominant spotting phenotype resulted from a nonsense mutation of Pax3 in Wbct mouse. SO - Jiangsu Journal of Agricultural Sciences. 2008. 24: 6, 857-861. 18 ref. PU - Jiangsu Academy of Agricultural Sciences LP - Nanjing CP - China AB - To study the heredity pattern and identify the mutation gene of a kind of white spot mouse (named Wbct) obtained by ethylnitrosourea (ENU) mutagenesis, wbct heterozygotes mice were mated with normal C57BL/6 (B6) mice. Their offspring with normal or mutation phenotype were registered respectively and x2 test was used to confirm the heredity pattern. Fine mapping was conducted by increasing (B6 x D2) x B6, N2 mice DNA sample and microsatellite marker. Based on fine mapping, the candidate gene Pax3 was found. Pax3 gene from wbct heterozygote gene was amplified by PCR followed by sequencing. Heredity test indicated that the white spot phenotype is controlled by a single dominant gene with 100% penetrance but was affected by genetic background. The homozygous mice could not survive. A c-to-a transversion at the 264th nucleotide in WbctM ORF introduced a pre-termination codon of amino acid and caused the phenotype al dominant white spot. CC - Animal Genetics and Breeding [LL240] CC - Molecular Biology and Molecular Genetics [ZZ360] MH - dominance MH - gene mapping MH - genetic markers MH - heterozygosity MH - microsatellites MH - mutagenesis MH - mutations MH - open reading frames MH - phenotypes MH - mice BT - Muridae BT - rodents BT - mammals BT - vertebrates BT - Chordata BT - animals BT - small mammals BT - eukaryotes ID - heredity ID - minisatellites ID - offspring ID - ORFs LG - Chinese SL - English PT - Journal article SS - 7B SS - 0A SS - CA SS - BE SS - ZA SS - ZS UR - http://www.jaas.ac.cn IS - 1000-4440 UP - 20090408 YR - 2008 <86> VN - Ovid Technologies DB - CAB Abstracts UI - 20093086462 AU - Fu ZeHong AU - Cao ShaoXian AU - Qian Yong AU - Zhang Jun AU - Zeng JianHua AU - Meng ChunHua AU - Zhong Sheng AU - Liu TieZheng IN - Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. MA - caosx@263.net TI - Polymorphism analysis of MSTN gene digested with Dra I in eight goat and sheep populations. SO - Jiangsu Journal of Agricultural Sciences. 2008. 24: 6, 853-856. 10 ref. PU - Jiangsu Academy of Agricultural Sciences LP - Nanjing CP - China AB - To investigate the influence of MSTN gene on the growth trait of sheep and goats, the polymerase chain reaction (PCR) products of MSTN gene 5'UTR in eight goat and sheep populations were digested with restriction enzyme Dra I and the polymorphism sites were detected. The results showed that only BB genotype distributed in detected sheep populations, however, three kinds of genotypes (AA, AB, and BB) distributed in goat populations. In Boer goat and the Yangtze River delta white goat, the distribution of AA genotype was highest, that of AB genotype was in the middle, and that of BB genotype was use lowest. In Huanghuai goat, the distribution of all genotype was the highest, that of BB genotype was in the middle, and that of AA genotype was the lowest. These results afforded a good foundation for further study on the influence of MSTN gene on the growth trait of goats. CC - Animal Genetics and Breeding [LL240] CC - Molecular Biology and Molecular Genetics [ZZ360] MH - Boer MH - genes MH - genetic effects MH - genetic polymorphism MH - genotypes MH - growth MH - traits MH - goats MH - sheep BT - Capra BT - Bovidae BT - ruminants BT - Artiodactyla BT - mammals BT - vertebrates BT - Chordata BT - animals BT - ungulates BT - eukaryotes BT - Ovis LG - Chinese SL - English PT - Journal article SS - 7B SS - 0A SS - CA SS - BE SS - ZA SS - QV SS - ZS UR - http://www.jaas.ac.cn IS - 1000-4440 UP - 20090408 YR - 2008 <87> VN - Ovid Technologies DB - CAB Abstracts UI - 20093086461 AU - Ni YanXiu AU - Zhou JunMing AU - Zhang XueHan AU - Wen LiBin AU - LuLiXin AU - Guo RongLi AU - Yu ZhengYu AU - Mao AiHua AU - He KongWang AU - Lu ChengPing IN - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. MA - niyanxiu@yahoo.com.cn TI - The distribution of inosine-5-monophosphate-dehydrogenase gene in different serotypes of Streptococcus suis. SO - Jiangsu Journal of Agricultural Sciences. 2008. 24: 6, 848-852. 11 ref. PU - Jiangsu Academy of Agricultural Sciences LP - Nanjing CP - China AB - A pair of specific primers were designed and synthesized according to the full-length genomic sequence of IMPDH gene from GenBank. 34 strains of Streptococcus suis were sequenced and analysed. In addition to 17, 24, 32, 33, and 34, the other 29 serotypes were positive. Homology analysis showed nucleotide sequence homology were 88.3%-100.0% and the derived protein sequence homology were 94.7%-100.0%. It provides opportunities for further study on biological characteristics and functions of IMPDH. RN - 58-63-9 CC - Prion, Viral, Bacterial and Fungal Pathogens of Animals [LL821] CC - Molecular Biology and Molecular Genetics [ZZ360] CC - Genetics and Molecular Biology of Microorganisms [ZZ395] MH - amino acid sequences MH - genes MH - genomes MH - inosine MH - nucleotide sequences MH - serotypes MH - strains MH - Streptococcus suis BT - Streptococcus BT - Streptococcaceae BT - Lactobacillales BT - Bacilli BT - Firmicutes BT - Bacteria BT - prokaryotes ID - protein sequences LG - Chinese SL - English PT - Journal article SS - 7D SS - 0I SS - 0V SS - CA SS - VE SS - ZA SS - ZS UR - http://www.jaas.ac.cn IS - 1000-4440 UP - 20090408 YR - 2008 <88> VN - Ovid Technologies DB - CAB Abstracts UI - 20093086459 AU - Zhu HongXia AU - Chen XiaoMin AU - Fang Kun AU - Yu Liang IN - College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. TI - Spatial variation of nitrogen and available phosphorus in soil of Taihu Lake region. SO - Jiangsu Journal of Agricultural Sciences. 2008. 24: 6, 839-842. 15 ref. PU - Jiangsu Academy of Agricultural Sciences LP - Nanjing CP - China AB - The spatial variation of N and available P in soil profiles of farmland soils (two main paddy soils, Whitish soil and Wushan soil) in the Lake Taihu Region (China) were investigated. It was found that the NH4+-N and NO3--N in the soil profiles gradually decreased from surface soil layer to bottom soil layer. NH4+-N content was stable below the surface soil. In Whitish soil, there was increasing accumulation of available P with increase in soil depth, which reached the maximum amount at a depth of 12-20 cm. In the Wushan soil, available P decreased with soil depth. The two soils showed little difference in the pH value. RN - 7727-37-9 CC - Soil Chemistry and Mineralogy [JJ200] CC - Soil Fertility [JJ600] MH - agricultural soils MH - ammonium nitrogen MH - nitrate nitrogen MH - nitrogen MH - paddy soils MH - phosphoramidate insecticides MH - soil depth MH - soil pH MH - soil types MH - spatial variation MH - China BT - East Asia BT - Asia BT - Developing Countries ID - ammonia nitrogen LG - Chinese SL - English PT - Journal article SS - 0S SS - 0E SS - CA SS - AG SS - PE SS - EC SS - SO SS - ZS UR - http://www.jaas.ac.cn IS - 1000-4440 UP - 20090408 YR - 2008 <89> VN - Ovid Technologies DB - CAB Abstracts UI - 20093086458 AU - Zhang YongChun AU - Wang JiDong AU - Nie GuoShu AU - Ding HanPing AU - Sun Li AU - Xu XianJu AU - Wang ZhiMing IN - Institute of Agricultural Resource and Environmental Sciences, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. MA - yczhang66@sina.com TI - Effect of mechanized straw application on crop yields and soil labile carbon content. SO - Jiangsu Journal of Agricultural Sciences. 2008. 24: 6, 833-838. 12 ref. PU - Jiangsu Academy of Agricultural Sciences LP - Nanjing CP - China AB - Field experiment were established to study the effect of mechanized straw application on the yields of rice and wheat and soil labile carbon content variation in Tai lake region in China. The plant height, shoot and root biomass of wheat seedlings on average were increased by 5, 16 and 13.0%, respectively, after 3 years of continuous application of straw. Rice and wheat yields were 2.1-8.6% and 5.1-13.0%, respectively, higher in straw-applied fields than in the field with no application of straw (control). Soil organic matter (OM) content was increased and each OM ingredient reacted discrepantly to straw application. Labile OM increased the most, being 2.5 times higher than the initial soil, while the total OM increased the least, the increasing rate was 26% only. Compared with carbon management index (CMI) of the initial soil, the soil CMI of half-amount and total-amount of straw application was increased by 1.52-1.94 and 1.22-1.99, respectively. Compared to the control, the soil CMI of half-amount and total-amount of straw application was increased by 67.7 and 72.0%, respectively, indicating total-amount application of straw was more effective in improving the soil CPMI. The soil CMI in rice season was significantly lower than that of wheat season. Analysis on the correlation between crop yields and soil OM ingredients suggested that the labile OM and CMI were better related to crop yield than total OM. Consequently, the soil labile OM and CMI are better evaluation indexes than the total OM in view of soil productivity. RN - 7440-44-0 CC - Field Crops [FF005] CC - Plant Production [FF100] CC - Soil Chemistry and Mineralogy [JJ200] CC - Plant Wastes [XX200] MH - carbon MH - correlation analysis MH - crop yield MH - mechanical methods MH - plant height MH - rice MH - rice straw MH - roots MH - shoots MH - soil organic matter MH - straw MH - wheat MH - wheat straw MH - Oryza MH - Oryza sativa MH - Triticum MH - Triticum aestivum MH - China BT - East Asia BT - Asia BT - Developing Countries BT - Oryza BT - Poaceae BT - Cyperales BT - monocotyledons BT - angiosperms BT - Spermatophyta BT - plants BT - eukaryotes BT - Triticum ID - organic matter in soil ID - paddy LG - Chinese SL - English PT - Journal article SS - 0S SS - 0Q SS - 6T SS - 7U SS - CA SS - CR SS - EC SS - SO SS - ZS UR - http://www.jaas.ac.cn IS - 1000-4440 UP - 20090408 YR - 2008 <90> VN - Ovid Technologies DB - CAB Abstracts UI - 20093086457 AU - Chen LiuGen AU - Zhang BaoSheng AU - Zhuang HengYang AU - Xue XinHong IN - Institute for Agricultural Resources and Environments, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. MA - chenliugen@sina.com TI - Effects of protective tillage of paddy fields on soil fertility under different rice growing stage in Taihu Region. SO - Jiangsu Journal of Agricultural Sciences. 2008. 24: 6, 826-832. 11 ref. PU - Jiangsu Academy of Agricultural Sciences LP - Nanjing CP - China AB - An experiment was carried out since 2004 in Taihu Lake District, Jiangsu, China, with 3 cropping patterns (wheat-rice, ryegrass [ |
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